Greetings, Can anyone tell us if (IgG) anti glycophorin A antibody will cause substantial changes in light scatter (particularly forward scatter) of (human) RBC? We seem to see decreased forward scatter to the point of many of the RBC almost being off scale to the left, and increasing the FSC gain doesn't really help to bring these back on scale. This was just observed today and the investigators will have to go back and look at the cells microscopically after staining but I thought I would ask the group. Should they leave out added Ca and Mg in their binding and wash buffer, i.e., are these (Ca/Mg)more of a problem with antibody staining of RBC than lymphocytes? Thanks for any information. Ray Hester College of Medicine Univ. of South Alabama rhester@jaguar1.usouthal.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:09 EST