Hi Margherita, This is an area where definitions are not clear. Based on my studies in mice, I can tell you the following. In the spleen, F4/80 stains most tissue macrophages, there are other antibodies that identify subpopulations in the spleen, mostly based on immunohistochemistry studies. However in peripheral blood, F4/80 stains ~0.5% of CD45+ cells. In contrast there are ~10% of CD45+ cells that are CD11b+ and negative for the granulocyte marker Gr-1(Ly6G). Granulocytes in peripheral blood are quite bright for CD11b by flow and will need to be accounted for in acute inflammation. Most of the 11b+, Gr1- cells are assumed to be monocytes but CD11b is also supposed to be expressed on NK and some other cell types. You may need to do some pilot studies with a series of antibodies with and without a positive control inflammatory stimulus to see which antigens are expressed where. CD14 is supposed to be upregulated by activation and might be useful for your project although I have not looked at it. Hope this helps. -Jim Weaver ************************************************* * * * James L. Weaver Ph.D. * * Division of Applied Pharmacology Research * * Office of Testing & Research * * CDER MOD-1, FDA * * 8301 Muirkirk Rd, Laurel MD 20708 * * * * Phone: 301-827-8237 * * Fax: 301-594-3037 * * Email:WEAVER@CDER.FDA.GOV * * * * This email is my personal communication and * * is not in any way official U.S. FDA Policy * ************************************************* * -----Original Message----- * From: margherita.blasco@katamail.com * [mailto:margherita.blasco@katamail.com] * Sent: Monday, April 29, 2002 8:13 PM * To: Cytometry Mailing List * Subject: Monocytes and macrophages * * * * Hello, * * I am in search of markers for murine monocyte/macrophages. I * do not have experience * in flow cytometry, so I looked in the literature and my * choice would be the F40/80, * which should stain both monocytes and macrophages, and * CD11b. Is this correct? * I need to follow monocyte migration (and differentiation in * macrophages?) in inflammatory * disease of the muscle, and I would like to have markers that * make me detect both these * cell types. * * Thank you for any input, of which I am in very much need! * * Margherita * * * __________________________________________ * * * Fai i tuoi acquisti su www.kwshopping.it * *
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