Hi, You could try using an anti-phospho histone H3 antibody, which is a marker of mitosis. You may be able to tell if a particular phase of mitosis is being affected from microscopic observation of the staining. See the references below for protocols and images: Juan et al., Cytometry 32:71-77, 1998 Widrow and Laird, Cytometry 39(2):126-30, 2000 Abu-Absi and Srienc, J Biotechnology 95(1):63-84, 2002 Nick Abu-Absi Process Development Scientist Chiron Corporation 4560 Horton St. M/S M-200 Emeryville, CA 94608 Lab: (510)923-7421 Fax: (510)923-4116 -----Original Message----- From: HILL, RON [R&D/0111] [mailto:ron.hill@sugen.com] Sent: Wednesday, August 28, 2002 9:53 AM To: cyto-inbox Subject: Measirung Cell Cycle by Cytometry Hi all, We are interested in analyzing a phenotype that has a blockage in mitosis. Can anyone suggest reagents and protocols to determine what specific phase of mitosis is being impacted. Thanks in advance. Ron Hill Ronald J. Hill, Ph.D. Sr. Group Leader, Research Sugen, Inc. 230 East Grand Ave. South San Francisco, CA 94080 ron-hill@sugen.com tel: 650-837-3797 fax: 650-837-3313
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