Woong-Ki, When I was doing a lot of flow on monocytes and granulocytes, it was my understanding that unlike lymphoid cells, myelocytic differentiation markers are not exclusively expressed on one population vs. another. Thus, unlike CD19 on B cells vs. CD3 on T cells, CD14 is highly expressed on monocytes and expressed on lower levels on granulocytes, and the converse is true for CD15. That being said, differential levels of expression of these two markers should allow very good discrimination between the two cells. In addition, CD16 is very brightly expressed on all granulocytes, and is at lower levels on a subset of monocytes. I think that there are differences between the isoform of CD16 expressed on these cells, the granulocytes expressing the PI glycolinked form recognized by 3G8, and monocytes expressing another form. (I am not sure of this, you will need to check it out). According to the BD chart, CD80 and CD86 are expressed on monocytes and not granulocytes. ron on 10/4/02 12:39 PM, wkim1@caregroup.harvard.edu at wkim1@caregroup.harvard.edu wrote: > > Dear Flowers, > > We are looking for a (or the) marker for granulocytes in human or monkey > PBMC to distinguish monocytes from them. Gating monocytes in our Ficolled > PBMC based upon FSC/SSC plot alone does not do the job (cannot exclude > contaminating granulocytes, I believe). The purpose of our PBMC prep is not > to eliminate granulocytes, but to evaluate granulocytic contribution to > monocyte population (i.e. enumerating granulocytes vs. monocytes). To serve > this purpose, we need to have a pan-marker for human and monkey granulocytes > if there is such an antibody. I would very much appreciate for your help > and expertise. > > Sincerely, > > Woong-Ki Kim, Ph.D. > BIDMC > Div Viral Pathogenes > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:26 EST