Dear Flowers, We are looking for a (or the) marker for granulocytes in human or monkey PBMC to distinguish monocytes from them. Gating monocytes in our Ficolled PBMC based upon FSC/SSC plot alone does not do the job (cannot exclude contaminating granulocytes, I believe). The purpose of our PBMC prep is not to eliminate granulocytes, but to evaluate granulocytic contribution to monocyte population (i.e. enumerating granulocytes vs. monocytes). To serve this purpose, we need to have a pan-marker for human and monkey granulocytes if there is such an antibody. I would very much appreciate for your help and expertise. Sincerely, Woong-Ki Kim, Ph.D. BIDMC Div Viral Pathogenes
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