> Group, Does anyone have a suggestion for the best laser line to use for >sorting cells expressing (only) dsRed? Thanks, Phil Marder Hi Phil, The excitation profile for dsRed peaks at 553nm but has shoulders at 488nm and 526nm. We have excited and sorted dsRed containing cells using the 488nm line. If your cells have a reasonable amount of protein, that should be OK. If it is weakly expressing, you may have more success with eg a green HeNe. Good luck, Derek ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 London Research Institute, e_mail: derek.davies@cancer.org.uk Cancer Research UK mobile: 07790 604112 44 Linolns Inn Fields, London, UK. Web Page: http://sci.cancerresearchuk.org/axp/facs/davies/index.html In tenebris lux *************************************************************************
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