Re: sorting dsRed

From: William Telford (TelfordW@mail.nih.gov)
Date: Fri Jul 26 2002 - 22:14:17 EST 




Hi Phil...


Although a 488 nm line will work for DsRed, it prefers a green or yellow
line.  You can tune most water-cooled (and some air-cooled) argon
lasers to the 514.5 for enhanced excitation of DsRed, DsRed2 and
HcRed.  If you have access to a mixed gas or krypton laser, the 520,
530 and 568 nm lines from these sources work even better (particularly
for HcRed).  If you use the 568 nm yellow line, make sure your DsRed
bandpass filter is blocked for 568 nm light (or use a 568 nm restriction
bandpass along with your filter). 


We (in collaboration with Teresa and Bob Hawley at the ARC Holland Lab)
have some comparative excitation data for DsRed at all of the above
wavelengths posted on our website
(http://home.ncifcrf.gov/ccr/flowcore/DsRed.htm).


Good luck,


Bill Telford

NCI-NIH


At 11:17 AM 7/26/2002 -0500, Phil Marder wrote:


Group,

 

Does anyone have a suggestion for the best laser line to use for sorting
cells expressing (only) dsRed?

 

Thanks,

Phil Marder




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