Dear Flow Users A patient was referred to the Haematology Department of Fremantle Hospital on 20/09/02 after being treated for a suspected CLL/prolymphocytic leukaemia with a WCC of 200 X10^9/L on 02/08/02. Prednisolone 50mg was given for approximately one week and Chlorambucil 6mg for 7 days. The WCC was 12.0 x 10^9/L on presentation at Fremantle Hospital. Morphologically the white cells appeared to be T cell prolymphocytes. Bone marrow and peripheral blood immunophenotyping was done. The abnormal cells made up 60% of the bone marrow cells. They were gated using CD45-PC5/LSS and had forward and side scatter characteristics of lymphocytes. They were positive for CD7, CD11b, CD13, CD33, CD34, CD45, CD117, and weakly positive for HLADR and negative for CD2, CD3, CD4, CD5, CD8, CD10, CD14, CD15, CD16+CD56, CD19, CD20, CD61, CD71, CD79b, cCD3, cMPO, and cCD79a. Comments would be greatly appreciated. Regards Lynette Tytherleigh Haematology Department Fremantle Hospital Alma St Fremantle 6959 Western Australia Phone: 08 94312459 Email: Lynette.Tytherleigh@health.wa.gov.au
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