Hello colleagues, Does anyone know of markers that one can use for purifying epithelial cells and lymphocytes from murine gut using sorters or magnetic beads? Presumably for lymphocytes one can use antibodies to the usual markers (CD8, anti-gamma delta etc). What about epithelial cells? Most of the methods I have used involve collagenase treatment and percoll gradients which can have both yield and purity issues. I was wondering if there are better methods for purifying cells from guts? Thanks. Roli The information contained in this email is intended for the personal and confidential use of the addressee only. It may also be privileged information. If you are not the intended recipient then you are hereby notified that you have received this document in error and that any review, distribution or copying of this document is strictly prohibited. If you have received this communication in error, please notify Celltech immediately on: +44 (0)1753 534655, or email "is@celltech.co.uk"
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