Re: tetramer staining after vaccination

From: mandy cromwell (mandy_cromwell@hms.harvard.edu)
Date: Mon Aug 19 2002 - 12:00:20 EST 

Stoyan,

There are a number of papers describing tetramer and other
antigen-specific T cell responses in macaques immunized with
candidate AIDS vaccines (ie:SIV), before virus challenge. I don't
know if anything similar has been published for human subjects. The
most commonly used methods for measuring antigen-specific T cells
responses in immunized macaques are tetramer analysis, intracellular
cytokine staining, and ELISPOT.
For some examples, see:

Shiver, J.W. et al, Nature 415,331 (2002)
Amara, R.R. et al, Science 292, 69 (2001)
Barouch, D.H. et al, Science 290, 487 (2000)

I hope this is helpful,

Mandy Cromwell


Dear colleagues,

>I am not new in cytometry but I need a little help in tetramer
>staining (or other in-
>vitro methods for measuring antigen specific T-cells). So, I work in
>neuroendocrine
>laboratory and we need an appropriate model for our research. This
>could be a vaccinated
>human against a virus disease. I would like to ask if it is possible
>to detect any
>antigen specific T-cells after any kind of vaccination. I try to
>find some articles but
>the most concern response after some disease.
>I will appreciate any articles and advices concerning that topic.
>Thank you in advance
>
>Stoyan
>Klinische Forschergruppe Neuroendokrinologie
>Medizinische Universit”t Lžbeck
>Ratzeburger Allee 160
>Haus 23a
>23538 Lžbeck
>Tel.: +49 (451) 500-3644
>Fax.: +49 (451) 500-3640



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Mandy Cromwell, Ph.D.
Department of Immunology
New England Regional Primate Research Center
Harvard Medical School
One Pine Hill Drive
Southborough, MA 01772
(508)624-8022
mandy_cromwell@hms.harvard.edu

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