Dear Fellow Flowers, Does anyone have any information about monomeric avidin? (or monomeric streptavidin) i.e. an avidin that can bind just one biotin molecule? We are constructing a ligand for a T cell surface molecule which will have one biotin molecule attached. If there was a streptavidin-PE conjugate available that could bind just one biotin, then we might be able to accurately quantify how much ligand was bound using flow cytometry. Thanks for any information you might have. Ed Palmer ______________________ Ed Palmer M.D. Ph.D. Laboratory of Transplantation Immunology and Nephrology Department of Research University Hospital-Basel Hebelstrasse 20 CH-4031 Basel, Switzerland e-mail: ed.palmer@unibas.ch FAX: + 41 61 265 34 20
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