monocyte isolation

From: Calin Tatu (cta@med.unc.edu)
Date: Thu Jul 25 2002 - 11:21:49 EST

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Dear Flowers,

Does anyone have a reliable protocol for density gradient separation of human monocytes from whole peripheral blood? We have tried several Fycoll densities and osmolarities but none seems to work. We have been using magnetic isolation on MACS but we are looking for a more expedient and cheaper way to get pure (i.e. >90%) monocytes.
    Any reply would be greatly appreciated.

Thank you,

Calin Tatu.

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