RE: monocyte isolation

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Dear Calin

 Unfortunately I can't be of help regarding monocyte isolation	BUT I would
be VERY interetsed in learning more about  magnetic isolation of Macs

Many thanks in advance

 Annette
-----Original Message-----
From: Wolber, Fran [mailto:F.M.Wolber@massey.ac.nz]
Sent: Sunday, July 28, 2002 1:46 PM
To: cyto-inbox
Subject: RE: monocyte isolation


Calin

Try looking up a paper from around 1990-ish, in J Imm Methods, by Elizabeth
Denholm, on isolating human monos using a modified Percoll gradient.

Fran Wolber
Institute of Food, Nutrition and Human Health
Massey University

-----Original Message-----
From: Calin Tatu [mailto:cta@med.unc.edu]
Sent: Friday, 26 July 2002 4:22 a.m.
To: cyto-inbox
Subject: monocyte isolation

Dear Flowers,

Does anyone have a reliable protocol for density gradient separation of
human monocytes from whole peripheral blood? We have tried several Fycoll
densities and osmolarities but none seems to work. We have been using
magnetic isolation on MACS but we are looking for a more expedient and
cheaper way to get pure (i.e. >90%) monocytes.
    Any reply would be greatly appreciated.

Thank you,

Calin Tatu.

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