Calin
Try looking up a paper from around 1990-ish, in J Imm Methods, by Elizabeth Denholm,
on isolating human monos using a modified Percoll gradient.
Fran Wolber
Institute of Food, Nutrition and Human Health
Massey University
-----Original Message-----
From: Calin Tatu [mailto:cta@med.unc.edu]
Sent: Friday, 26 July 2002 4:22 a.m.
To: cyto-inbox
Subject: monocyte isolation
Dear Flowers,
Does anyone have a reliable protocol for density gradient separation of human monocytes
from whole peripheral blood? We have tried several Fycoll densities and osmolarities
but none seems to work. We have been using magnetic isolation on MACS but we are
looking for a more expedient and cheaper way to get pure (i.e. >90%) monocytes.
Any reply would be greatly appreciated.
Thank you,
Calin Tatu.
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