differences in blood collection

From: MIGUEL-ANGEL PERALES (mperales@OPAL.TUFTS.EDU)
Date: Thu Mar 07 2002 - 16:03:50 EST


Not exactly a flow question, but somewhat relevant: We recently did a
study looking at T cell responses to a vaccine by Tetramer. Some
patients had green tops collected and others underwent leukapheresis.  It
seemed that T cell responses were higher in green top samples.  We didn't
compare the 2 methods in the same patient however.  Has anyone had a
similar experience or tested this?  Also, has anyone compared T cell
reponses in samples collected in green top vs, CPT tubes.

Thanks,

Miguel Perales



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