Dear Flower, If I understand well you are in search of TrinitroPhenyl (TNP) to conjugate to a protein. Then I would suggest the activated form TrinitroPhenyl benzene Sulfonate (TNBS), also named Picrylsulfonic acid . You can find it in Sigma , ref P2297. In case you want to do immunological detection, you may appreciate to know that there is a very high cross-reactivity of anti-DNP ( DinitroPhenyl ) antibodies against TNP-coupled proteins (at least in my personnal (old) experience). Thus, depending upon your objective, you may also envisage to conjugate your antigen with DNP. Then you have many options for coupling DNP on amines (DNP-succinimide see DNP-X-SE at Molecular Probes) or even may-be onto -SH moieties (DNP-Cysteine in Sigma). Hope that helps. Philippe Poncelet, PhD Director, Research and Technology BioCytex 140, Chemin de l'Armée d'Afrique 13010 Marseille France Tel: +33 (0) 4 96 12 20 40 Fax: +33 (0) 4 91 47 24 71 www.biocytex.fr Tony Schountz <tschount@mesastate.edu> le 13/11/2002 01:22:21 Pour : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc : Objet : TNP conjugation I'm in need of TNP to conjugate to an antigen, but I can't seem to locate it in any of the major catalogs (Sigma, et al.). Could someone point me to a company that sells hapten-grade TNP? Thanks, Tony -- Tony Schountz, Ph.D. Dept. of Biology Mesa State College Grand Junction, CO 81506 http://www.mesastate.edu/~tschount/
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