Simon, we have started to use CBA from BD for quantifying inflammatory cytokines from pediatric patients some time ago and our experience is very positive. We have run samples vs. standard ELISAs of IL6, IL8 and IL10 and the correlations were always >0.93. I have presented these data on ISACXXI. For us this system is very important, because we deal with samples from neonates where we always run into problems with standard ELISAs because of too low sample volumes. We tried CBA on our Laser Scanning Cytometer and it works there as well and there should be no problem to run it on any other machine. You only have set up your system once so that the low and high standards are in scale and adjust the compensation (this takes 5min). I hope this helps, Attila Tarnok Simon Watson schrieb: > Hi Everyone Has anybody used the BD Cytokine Bead assay, which uses > antibody-labelled fluorescent beads to detect cytokines? I've seen a > demo on a FACS Caliber and it looks pretty impressive, especially the > Excel-based analysis software. BD obviously have set up their system > to work best on one of their machines and supply instructions for > compensation, thresholds etc. Has anyone tried the CBA system on a > Coulter XL or another type of flow cytometer. I can't see any reason > why it wouldn't work but imagine you'd have to work out your own > settings empirically. Please advise! Best wishes Simon > Watson Phagocyte LabMRC Centre for Inflammation ResearchUniversity of > EdinburghUK
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