If I remember my inorganic chemistry, Lithium has a much larger ion, maybe similar to size of Calcium? whatever the reason is, cells exposed to lithium are reduced down to NO reactivity in any functional study. Maciej on 6/17/02 10:42 AM, Frank Mattes at list_server2001@yahoo.com wrote: > > Dear flow-cytometry reader, > > not strictly a flow question. I'm isolating PBMCs from > Li-heparin blood for an ongoing study. PBMCs get > frozen and stained later for surface markers, > including tetrameric complexes. > In addition I'm using the cells for functional studies > (ELISpots, CD8 response). Because Li-Heparin is used > routinly when collecting blood in our hospital, we > used these type of blood so far. However, someone > mentioned that Li will affect the funtional capacety > of the cells,and Na-Heparin is superior. > > I'm wondering if someone has expirience or had already > compared Li/Na heparin as a preservative. > > Many thanks > > Frank Mattes > Department of Virology > Royal Free Hospital > London > f.mattes@ucl.ac.uk > > __________________________________________________ > Do You Yahoo!? > Yahoo! - Official partner of 2002 FIFA World Cup > http://fifaworldcup.yahoo.com >
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