We find that the cells are not good for cytogenetics studies (won't divide) after lithium heparin and that the viability can be less on occasion. We always specify sodium heparin. Maryalice Stetler-Stevenson, M.D., Ph.D. Chief, Flow Cytometry Unit Laboratory of Pathology, NCI, NIH Sometimes you're the windshield, sometimes you're the bug. > ---------- > From: Frank Mattes > Sent: Monday, June 17, 2002 10:42 AM > To: Cytometry Mailing List > Subject: Na-heparin / Li-heparin for isolating lymphocytes > > > Dear flow-cytometry reader, > > not strictly a flow question. I'm isolating PBMCs from > Li-heparin blood for an ongoing study. PBMCs get > frozen and stained later for surface markers, > including tetrameric complexes. > In addition I'm using the cells for functional studies > (ELISpots, CD8 response). Because Li-Heparin is used > routinly when collecting blood in our hospital, we > used these type of blood so far. However, someone > mentioned that Li will affect the funtional capacety > of the cells,and Na-Heparin is superior. > > I'm wondering if someone has expirience or had already > compared Li/Na heparin as a preservative. > > Many thanks > > Frank Mattes > Department of Virology > Royal Free Hospital > London > f.mattes@ucl.ac.uk > > __________________________________________________ > Do You Yahoo!? > Yahoo! - Official partner of 2002 FIFA World Cup > http://fifaworldcup.yahoo.com > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:13 EST