Dear flow-cytometry reader, not strictly a flow question. I'm isolating PBMCs from Li-heparin blood for an ongoing study. PBMCs get frozen and stained later for surface markers, including tetrameric complexes. In addition I'm using the cells for functional studies (ELISpots, CD8 response). Because Li-Heparin is used routinly when collecting blood in our hospital, we used these type of blood so far. However, someone mentioned that Li will affect the funtional capacety of the cells,and Na-Heparin is superior. I'm wondering if someone has expirience or had already compared Li/Na heparin as a preservative. Many thanks Frank Mattes Department of Virology Royal Free Hospital London f.mattes@ucl.ac.uk __________________________________________________ Do You Yahoo!? Yahoo! - Official partner of 2002 FIFA World Cup http://fifaworldcup.yahoo.com
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