Mario Roederer wrote- >The cheap and easy solution (depending on your hardware) to avoid charge >accumulation for one-way sorting is to push the central waste stream >collection off to the side (Dick Stovel taught us to use a chopped-off >yellow tip of the right length, wedging it in to push the collector >over)--set the voltage balance to push the central stream into the waste >collection, and the sort stream to be absolutely vertical (i.e., no net >charge). You can aim a drop into a very small area using this method, and >for large sorts, there's no charge accumulation. > >It would be nice if manufacturers would make the necessary accomodations >to make "vertical" sorts easier to set up; in my opinion, there's no >reason it shouldn't be done anytime you do a one-way sort! If I recall correctly, the single cell sort accessories for droplet sorters do vertical sorts, and deflect the central stream (i.e. charge all the droplets except the ones containing the desired cells). -Howard
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