In general the azide is added as a metabolic inhibitor to prevent capping,
patching and endocytosis of cell surface markers following staining. This
does not occur with all cell surface antigens but can be a problem for
staining. If you want to eliminate the Azide, keeping cells constantly on
ice accomplishes the same thing.
Alan
==========================
"Dr. Ashraf
Abdelhafez" To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
<asabdelhafez@ cc:
yahoo.com> Subject: Azide?
09/25/02 05:28
AM
Hi All:
Does anybody have a conclusive answer to why azide is added to staining
buffer. I have found information that says that it functions to inhibit
microbial growth. I have found that it can be used to stop reactions. I
have found people recommending not to use in certain experiment because it
can alter protein function. And I have found people saying that you do not
need to put if you are going to analyze the cells within hours. But I was
not able to find information on that exact reason for adding it to staining
buffer.
I would like to hear what you have. Only what you believe is a conclusive
answer is accepted.
Thanks,
Ashraf
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