FACS-analysis of muscle cells from tissue sections

From: u_pannasch@gmx.de
Date: Fri Jun 07 2002 - 10:21:38 EST


Hi,
I´m looking for a protocol for FACS-analysis of muscle cells from tissue
sections. I didn`t want to culture muscle cells because of changes of
extracellular matrix proteins (especially integrines). Is it possible to use cells from
tissue sections for FACS-Aanalysis.
Additional I need an monoclonal antibody against the integrine alpha 7 beta
1. I can´t find this type of antibody.
I´m grateful for every advice.
Thanks,
Ulli

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