Iaj,
We have found that if you acquire cells on a LogFL1(GFP) vs LogFL2 dotplot
and set the instrument settings, so that the cells fall in the first decade,
you can discriminate gfp +ve cells from background fluorescence by applying
some FL2-FL1 compensation. This should bring any gfp +ves down towards the
FL1 axis. If you keep the PMT settings for F1 and FL2 roughly equal you
probably find you may need to apply about 15% compensation. This works well
with cells but I can't say for protoplasts. Good luck.
Regards,
Jeff Barry
Flow Cytometry
Paterson Institute for Cancer Research
Manchester
UK
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