Vascular Smooth Muscle Cells

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Hello!!

I am a cardiovascular investigator trying to isolate vascular smooth
muscle cells from rat carotid arteries at as high purity as possible.
Unfortunately, a previous attempt showed that only 25% of isolated
cells  were alpha-actin positive after differentiation (heparin and 0.5%
sera for 4 days).  Other cells appear to be endothelial cells.

Does anybody have a good protocol for vascular smooth muscle cell
isolation??
In addition, do you have any cleaver way to use FACS to determine the
purity of the prep?

I thank you for your time and help on this.

Sincerely,

Ken

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