For those of you with an AutoMACS in your facility, please allow me to pick your brains. We have been performing either negative selections or positive depletions to enrich for monocytes on a routine and successful basis. Suddenly, our cells are dying after 1-2 days in culture. Using the same starting sample, cells isolated in parallel but by adherence are doing fine. Naturally, all reagents have been tossed and re-made but still no change. Additionally, there have been no changes to the AutoMACS cleansing reagents (just EtOH and bleach as recommended by Miltenyi) and the instrument is clean as a whistle. Any suggestions would be appreciated. I know this is vague but I'm crossing my fingers that someone out there can shed some light. Elizabeth Kraus B.I.I.R. Dallas, TX.
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:03 EST