NK cytotoxicity assay with human whole blood

From: Lam, Lee (lxl7@CDC.GOV)
Date: Tue Jun 25 2002 - 10:49:56 EST


Dear all,
Our lab would like to use flow cytometry for the measuring natural killer
cell cytotoxicity. Does anyone have a working protocol that can share with
us? Also, could we use 24 hrs. old human whole blood instead of PBMC for the
assay? It will be really appreciated if you can provide us with valuable
pointers.  Thanks in advance.
	Lee Lam
	CDC
	Atlanta, Georgia
	Phone: 404-639-2725
	Fax:	 404-639-4838
	E-mail:  LXL7@CDC.GOV <mailto:LXL7@CDC.GOV>



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