Re: granzymes Flow and ELISPOT and "free" advice

From: Mario Roederer (roederer@drmr.com)
Date: Tue Aug 13 2002 - 15:44:10 EST


At 12:52 PM +0200 8/13/02, Voorn, J. wrote:
>I am happy to inform you that we have been able to make the first and so far
>only monoclonal antibodies against native human granzymes which can be used
>in different immune assays.

This is a bit of an overstatement (and, for my taste, a bit too
commercial for this list).

Caltag has anti-granzyme antibodies, in fact the only ones that we've
found which cross-react with rhesus macaque.

We've extensively used the PharMingen antibodies against granzyme A,
B, and perforin.  In fact, we've used these simultaneously in
multicolor experiments (each conjugated to a different color)--they
work great.

We have also used the anti-granzyme antibodies made by CLB with good success.

mr



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