Dear Mark, I am happy to inform you that we have been able to make the first and so far only monoclonal antibodies against native human granzymes which can be used in different immune assays. For flow cytometry we made a PE-conjugated antibody against granzyme B, for granzyme A you can use our biotin conjugated antibody. So far most studies only focus on Granzyme B, although there is evidence that granzyme-A can induce apoptosis through a delayed pathway or as recently published it can even be depending on the target cell, Pardo J., Eur. J. Immunol.2002.32:1980-1985. I can not help you with an antibody for perforin but this will be easier to find. Please note that the function of perforin is now known to be involved in the delivery of the actually apoptotic granzymes and thus less relevant to study for CTL-activity, S. Metkar, Immunity, 16, 417, March 2002. On our web site www.clb.nl <http://www.clb.nl> (go to reagentia) you can find product information, protocols, example dot plots and a list of references. Also for these products we have free samples to test. The latest news on the field of our granzyme antibodies is the development of an ELISPOT kit, expected for release in November 2002. It is evident that ELISPOT is getting more and more used since it is easier to run many samples and much faster but comparable sensitive to flow. To evaluate our kits we are looking for test sites, studies with relevant patient groups etc. no ELISPOT experience required! Best regards, John John Voorn Sanquin Reagents Product manager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: Mark Pavlick [SMTP:MPAVLICK@niaid.nih.gov] Sent: Friday, August 09, 2002 3:21 AM To: Cytometry Mailing List Subject: AB's for human perforin, granzymes 8/8 List members, I want to stain human CD8 lymphocytes for the expression of perforin and granzymes A and B. I'd appreciate any references to currently available antibodies for this purpose. Thanks in advance. Mark Pavlick mvp@nih.gov
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