Hello everyone, First let me thank everyone for all the responses to my question regarding mast cell degranulation detection by FACS. I am in the process of trying several of your suggestions and tracking down the mentioned references. Of course I have another question... The researcher working with these cells wanted to know if any of you had a standard degranulation protocal that has worked well. And if so how much degranulation should we expect to see? 20% 100%?? We are working with a mouse cell line MCL 9 and a rat line RBL-2H3. Thanks again for all the help! Amy Amy Raber Flow Manager Athersys Inc. Cleveland, OH 44111 216-431-9900 ext 280 araber@athersys.com
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:29 EST