I have no specific experience with the clones you utilize. However, i have
no problem detecting follicular lymphomas or GC cells with CD10PE available
from Becton Dickinson.
F Preffer
At 08:44 AM 1/25/02 +0100, Jáksó Pál wrote:
>
>Dear flowers,
>
>One of our pathologists complained about our CD10 flow results of lymph
>node biopsies and fine needle aspirates, because they don't match with
>immuohistochemical staining which shows positivity of lymph nodes
>germinal center cells and follicular lymphoma while the flow shows
>negativity or very dim stain although this antibody works well in B-cell
>acut lymphoid leikaemia. Currently we use DAKO (clone: SS2/36) for flow
>and Novocastra CD10 (clone: 56C6) for immunohistochemistry. I think the
>different clones may give different staining.
>Novocastra does not produce fluorescent labelled CD10 for flow and I
>have to look for other sources to try different CD10 clones wich give
>comparable result with the immunohistochemistry in the case of lymph
>nodes.
>Has anybody experience on this ?
>
>Thanks,
>
>Pal Jakso
>
>University of Pecs
>Faculty of Medicine
>Dept. of Pathology
>7643, Hungary
>12. Szigeti str.
>
>
>
>
`````````````````````````````````````````````````
Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Room 7140
149 13th Street
Massachusetts General Hospital-East
Charlestown, MA 02129
v(617) 726-7481 fax (617) 724-3164
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