I would assume that any of the TOTO or TO-PRO series of dyes would have this property unless they entered the cell due to an endocytic process (some dyes of the TO-PRO series also enter apoptotic cells. The dyes are all essentially nonfluorescent until bound to nucleic acids. All are polycationic, which will affect the current used for electroporation. http://www.probes.com/handbook/sections/0801.html Probably best for 488-nm excitation would be TOTO-1 and for 633-nm excitation TOTO-3. Both are tetracationic. SYTOX Green is another alternative. FITC dextran would be a poor choice because it is sometimes less fluorescent in cells than outside (pH dependent fluorescence). Gisele Knowles wrote: > Hello colleagues- > Does anyone know of a product that can sit outside a cell and with either > electroporation or other such stressor, enter the cell and then fluoresce?? > I have this in my brain that such a thing exists and I'm concurrently trying > to avoid using fitc dextran altogether because I'm finding too much > non-specific fluorescence. Anyway all comments would be appreciated. > Thanks > > -- > Gisele Knowles > >>Director > Centre for Cytometry and Scanning Microscopy > Sunnybrook and Women's Research Institute > Room B 210 > 2075 Bayview Avenue > Toronto, ON > 416.480.6100x7282 (office) > x7284/5 (lab)
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