Dear all, not a strict flow question but a "sorting" question related to the use of magnetic beads. I am a little bit confused by all the systems/protocols currently available and would like to ask the following: 1. Magnetic vs para-magnetic. Although I think I understand the "phenomenology" behind that, and the fact that you need to use different apparatus, I wonder whether one system is better than the other. 2. Size of the beads. It looks to me that the smaller (50 nm range) paramagnetic beads made of biodegradable material may be more adequate for positive sorting. I have been told by people from Spherotec that larger beads (250 nm and up) perform better for negative selection. Any idea ? 3. The same antibody lot (DX5-biot from ebioscience) works better in a AUTOMacs than in a Dynabeads system for depleting mouse NK. Any idea why ? Other antibodies of course perform as well in both systems. 4. Does anyone has experience with beads (para or magnetic) that one can buy and easily couple to "home made" antibodies ? I know several companies offer the service, but I kind of like doing it myself. and finally 5. Are there any alternative to Myltenyi microbeads for using with an AUTOMacs system (sorry to bring this up, but these beads are very expensive !). Regards, Oberdan Oberdan LEO Laboratoire de Physiologie Animale Université Libre de Bruxelles Rue Profs Jeener et Brachet, 12 6041 Gosselies (BELGIUM)
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