Hi Flow People Could anyone answer my query: Does prophylactically administerd Anti-D immunoglobulin interfere with (inhibit) the binding of conjugated anti-D monoclonal antibody reagent on Rh D+ fetal red cells in Rh D negative maternal circulation? Surprisingly, I have not been able to find any reports that have specifically addressed this subject. Some simple in-vitro co incubation experiments would probably clarify the issue. Presumably the maternal reticulo-endothelial system is responsible for the removal of IgG (anti-D) coated fetal red cells. This mechanism of destruction is not instantaneous, raising the possibility that coated cells may be present in the specimen when performing this anti-D flow based assay for FMH. It is also unclear whether competition for the same red cell epitopes would occur by Anti-D immunoglobulin (polyclonal) and anti-D reagent (monoclonal antibody). Thanking you in anticipation of your responses Emanuel Raniolo Cell Analysis & Cryopreservation Laboratory Department of Haematology The Queen Elizabeth Hospital Division The Institute of Medical & Veterinary Science Woodville, South Australia
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