RE: Anti-CD3 and tetramer staining

From: McCloskey, Thomas (thomasm@nshs.edu)
Date: Wed Feb 13 2002 - 10:23:06 EST 

----Original Message-----
From: Michal Abel [mailto:abelm@necker.fr]
Sent: Tuesday, February 12, 2002 5:16 PM
To: cyto-inbox
Subject: Anti-CD3 and tetramer staining

Does anyone know if there is any inhibition of specific tetramer HLA
staining with anti-CD3 Ab (as lying closely in the immunological synapse)

   Hi Michal,

    There is published evidence for this:

Hoffmann et al, Competition of peptide MHC class I tetrameric complexes with
anti cd3 provides evidence for specificity of peptide binding to the tcr
complex, Cytometry 41:321-328, 2000.

Regards,
Tom

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