Calman, Zymed in California (800-874-4494) has biotinylated (and possibly fluorochrome conjugated) mouse monoclonal antibodies to human IgG4 that work great on immunoblots. I have not used them in flow, but if I had a need, this would be my first choice. Delynn Moss Centers for Disease Control Parasitic Diseases Division -----Original Message----- From: Calman Prussin [mailto:CPRUSSIN@niaid.nih.gov] Sent: Tuesday, February 12, 2002 9:50 AM To: cyto-inbox Subject: Abs to human IgG4- which? Dear flowees- I am trying something new, an immunoassay that requires an Ab to human IgG4. Since I usually use mouse Abs (and anti-mouse Ig secondaries) I am totally ignorant of who supplies the best antibodies to human IgG. My favorite source for goat anti-mouse polyclonals (SBA) does not carry goat anti-human IgG4. Questions: 1. Does anyone know of a great high signal-to-noise mAb or pAb to human IgG4 conjugated with biotin? to a phycobiliprotein? 2. PharMingen has 2 mAb clones, each of which recognizes a different epitope. I could use them both and increase my S/N. Any experience with these? Thanks, Calman _______________________ Calman Prussin Laboratory of Allergic Diseases NIAID/ National Institutes of Health
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