Abs to human IgG4- which?

From: Calman Prussin (CPRUSSIN@niaid.nih.gov)
Date: Tue Feb 12 2002 - 09:49:34 EST 

Dear flowees-

I am trying something new, an immunoassay that requires an Ab to human IgG4.
Since I usually use mouse Abs (and anti-mouse Ig secondaries) I am totally
ignorant of who supplies the best antibodies to human IgG. My favorite
source for goat anti-mouse polyclonals (SBA) does not carry goat anti-human
IgG4.

Questions:

1. Does anyone know of a great high signal-to-noise mAb or pAb to human IgG4
conjugated with biotin? to a phycobiliprotein?

2. PharMingen has 2 mAb clones, each of which recognizes a different
epitope. I could use them both and increase my S/N. Any experience with
these?

Thanks,

Calman
> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health

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