Endothelial cell detachment

From: Michael Herron (herro001@umn.edu)
Date: Thu Sep 05 2002 - 09:41:05 EST 

All,

I am working with a transformed endothelial cell line.  I have to
culture them to confluency before checking their fluorescence by FACS.
When I use trypsin/EDTA the cells come off nicely, but they remain in
large "sheets" that do not come apart with pipetting as many cells will.

Does anyone have any suggestions as to how to disaggragate these sheets
of cells?

Thanks,
Mike


--

     _____________________________________________________
    / Michael J. Herron,  U of MN, Dept. of Entomology   /
   / herro001@umn.edu                                   /
  / 612-624-3212 (lab) St. Paul, MN 55108              /
 /____________________________________________________/

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