FL1-FL3 compensation.

From: Dr. Ashraf Abdelhafez (abdelhafez-a.derma@mail.uni-wuerzburg.de)
Date: Tue May 21 2002 - 09:20:14 EST

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I have been doing 3 color analysis and am using a FITC staining antibody that is
staining very strongly and emmiting very strongly (up to the 4th log) in FL1. When I
have a FL1 vs FL3 plot, as the FL1 positivity goes higher down the X access the dots
curve upward in FL-3 reaching at the highest end of FL1 staining up to the 2nd log in
FL3. Is there a way to appropriately compensate FL3-FL1 since this is unavailable on
the standard FACScan software so that I do not see this and can accurately evaluate
the molecule actually stained for in FL3.

Thank you.

Ashraf Abdelhafez, M.B.B.S.
University of Würzburg Dermatology

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