> You can use FlowJo (www.flowjo.com/) for software compensation to deal > with this problem. For all of you using bright GFP, you will also notice > GFP signal in FL3 (in std. FACScan configuration) if you dont compensate. > > Good luck, > Rachel > > ======================================================= > Rachel M. Gerstein, Ph.D. > Department of Molecular Genetics and Microbiology > Graduate Program in Immunology/Virology > University of Massachusetts Medical School > 55 Lake Avenue North > Worcester, MA 01655-0002 > (508) 856-1044 > (508) 856-5920 (FAX) > > > ---------- > From: Dr. Ashraf Abdelhafez > Sent: Tuesday, May 21, 2002 10:20 AM > To: Cytometry Mailing List > Subject: FL1-FL3 compensation. > > > I have been doing 3 color analysis and am using a FITC staining antibody > that is > staining very strongly and emmiting very strongly (up to the 4th log) in > FL1. When I > have a FL1 vs FL3 plot, as the FL1 positivity goes higher down the X > access the dots > curve upward in FL-3 reaching at the highest end of FL1 staining up to the > 2nd log in > FL3. Is there a way to appropriately compensate FL3-FL1 since this is > unavailable on > the standard FACScan software so that I do not see this and can accurately > evaluate > the molecule actually stained for in FL3. > > Thank you. > > Ashraf Abdelhafez, M.B.B.S. > University of Würzburg Dermatology > > >
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