Hello All, I would like to ask those of you who are doing HIT Assay by flow the following: 1. What do you use as reagent platelets? Various articles indicated using PRP from fresh citrated blood from a normal. Is there a way to avoid drawing a normal with every batch of testing? We tried using platelet segment from blood bank platelet packs without much success, they appeared activated. 2. Is ABO compatibility a concern when adding patient serum/plasma to normal platelets? 3. What do you use for positive controls? Calcium ionophore, ADP? Any suggestions or help will be much appreciated! Nancy Gin United Hospital St. Paul, MN (651)220-8910 __________________________________________________ Do You Yahoo!? Sign up for SBC Yahoo! Dial - First Month Free http://sbc.yahoo.com
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