Hi there. Does anybody here has the answer to the following observation? We do proliferation analysis on mouse thymocytes (individual cells, no clumps), using BrdU labelling and a FACScan. We get on our histograms a nice peak corresponding to the non proliferating cells, and a few percents of cells proliferating, crawling out of the right base of that peak. Fine. The problem is that the large peak appears to be "moving" on the x axis from one sample to the other, in a mysterious manner, without any apparant correlation with animals, treatments, and so on. Something like 1 out of 5 measurements made the same day with the same setting shows that weird phenomenon... Any suggestion? Philippe.
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