Two excellent review articles on I/C and nuclear staining: Bauer KD, Jacobberger JW. Analysis of intracellular proteins. Methods Cell Biol. 1994;41:351-76. Review. No abstract available. PMID: 7532265 [PubMed - indexed for MEDLINE] Jacobberger JW. Stoichiometry of immunocytochemical staining reactions. Methods Cell Biol. 2001;63:271-98. PMID: 11060846 [PubMed - indexed for MEDLINE] Calman > ---------- > From: Lars Brichta > Sent: Monday, September 23, 2002 13:55 > To: Cytometry Mailing List > Subject: [localization within lymphocytes] > > > Dear all, > > is it possible to determine a protein in lymphocytes (isolated from whole > blood) which is localized in the cytoplasmatic space and the nucleus as > well? > I think probably it would be necessary to fix the cells and to use a > permeabilization agent which acts on both the cell membrane and the > nuclear membrane > as well? > > Does anybody know if there is a paper which gives any hints for such a > method or the use of a kit? > > Thanks a lot! > > Lars Brichta > Institute of Human Genetics > University Clinics Bonn, Germany > > > -- > Werden Sie mit uns zum "OnlineStar 2002"! Jetzt GMX wählen - > und tolle Preise absahnen! http://www.onlinestar.de > >
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