Re: Pig stem cell marker

From: Artur Summerfield (Artur.Summerfield@ivi.admin.ch)
Date: Thu Apr 18 2002 - 01:18:12 EST


"Jonni S. Moore" wrote:
>
> Hi All,
>
> Does anyone know of a source for pig stem cell markers (i.e. CD34) or
> if any other species cross reacts?
> Thanks for any help..
>
> Happy Spring from the very warm Northeast!!
>
> Jonni
> --
> Jonni S. Moore, Ph.D.
> Associate Prof. of Path. and Lab. Med.
> Director of Clinical and Research Flow Cytometry
> University of Pennsylvania Cancer Center
> University of Pennsylvania School of Medicine
> 203 John Morgan Bldg.
> Philadelphia, PA  19104-6082
> Phone:  215-898-6853
> FAX:    215-898-4227

Dear Jonni
We have used recombinant porcine SCF with a his-tag (produced in our
lab) followed by anti-his mAb to label C-kit+ cells by flow cytometry.
It is also possible to enrich these cells using MACS.
Please contact me if this might be of use for you.
Artur
--
 Dr. Artur Summerfield
 Institute of Virology and Immunoprophylaxis (IVI), Immunology
Department
 3147 Mittelhaeusern, Switzerland
 Tel (+41) 31 848 9377, Fax (+41) 31 8489222



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