--- Ana Radovanovic wrote: We are interested weather is possible do perform 3 colour analysis with APC or Cy-Chrome in the combination with FITC or PE due to spectral overlaping etc... --- end of quote --- Ana, On the FACSCalibur cytometer, it is possible to use FITC and PE and APC (as long as you have purchased the second 635nm red laser along with the 488nm laser). You can use FITC and PE and CyChrome if you only have the 488nm laser. You can compensate for the spectral overlap in both these cases and it works well. On the old FACScan cytometer, you can do FITC and PE and CyChrome, but you cannot do APC. So, with a 488nm laser you can do FITC and PE and CyChrome. If you have both a 488nm laser and a 635nm laser, you can do FITC and PE and CyChrome or APC. Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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