Hi, It's depend. You should have a second red laser to directly excite APC or Cy... built in the FACScalibur. If you have the standard 488nm blue laser only, you can use PE conjugated Cy dyes, eg. PE-Cy5 (PC5) conjugates or PerCp instead. Best regards, Gogy Your original message: > Dear flowers, > our institute is in the position of FacsCalibur Becton > Dickinson flow cytometer. We are interested weather is > possible do perform 3 colour analysis with APC or > Cy-Chrome in the combination with FITC or PE due to > spectral overlaping etc... > Thanks in advance. > > Ana Radovanovic > Institute for Oncology and radiology of Serbia > Laboratory for experimental immunology > Pasterova 14 > 11000 Belgrade > Serbia
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