Dear flow group, just a couple of questions for the experts.... CD10: We are currently using Caltag's CD10-FITC (clone 5-1B4) and seem to have problem with staining on some of our follicular lymphomas. Recently we had a case which was CD10-neg by flow but stained positive with Ventanna's IPOX CD10 (clone 56C6). It seems to be Caltag's only clone, Coulter has 3 clones (J5 which Coulter recommends, ALB1 and ALB2 from the Coulter/ Immunotech merger). Since everybody uses a diggerent terminology for their clones, can somebody tell me how they compare and which clone is the most wiedely used by the flow community? CD34: We seem to have non-specific staining of CD34-FITC (Caltag's clone 581 class III) on monos (we have a gate set around all the populations and have noticed this fairly frequently). In the past we have ignored this and we able to differentiate this from the real specific staining. However, we had a recent case of LPD/B with ?CLL/?HCL differentiation, where the monoclonal B-cells showed CD34 positivity (TDT-neg, CD20+, CD22+, bright lambda+). Because of the mature phenotype I was leaning towards ignoring it, but felt uneasy about totally ignoring it. I just happened to have the red CD34 from Coulter (same clone 581 class III) and those cells were negative! Has anybody else seen this? Much appreciated! Andrea Illingworth Dahl-Chase Flow Cytometry Bangor, Maine 207-990-4855
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:20 EST