Greetings, We have an investigator that needs the ability to fix human blood and stain later for surface and possibly intracellular antigens. A search of Medline came up with Jani et al, "Affordable CD4+ T cell counts by flow cytometry II. The use of fixed whole blood in resource-poor settings," Journal of Immunological Methods, Volume 257, Issues 1-2, Pages 145-154. This article talks about using a fixative called TransFix made by UK NEQAS before staining. A search of the UK NEQAS website did not find anything referencing TransFix. Does anyone have experience with this stuff or have some other magic potion that would do the trick? Thanks. Justin Fishbaugh Technical Director Flow Cytometry Facility Holden Comprehensive Cancer Center University of Iowa 48 EMRB Iowa City, IA 52242 (319) 335-8103 Tel (319) 335-8049 Fax http://www.medicine.uiowa.edu/flowcytometry/
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