Dear Flowers, We want to try to set up a protocol for detection of DNA damage and repair by flow cytometry. There are a lot of antibodies recognizing indirect markers such as phosphorylated form of histone H2AX (trevigen, upstate, ...) or DNA adducts i.e. 8-hydroxyl- deoxy guanosine (BD, trevigen). Has anyone already tried some of these products? Any suggestions about other flow cytometry tools to detect DNA damage and repair? Thanks, Simona Simona Ronzoni Dept. of Experimental Oncology European Institute of Oncology via Ripamonti, 435 20141 Milan Phone: +39 02 57489880 Fax: +39 02 57489851 --
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