Re: FITC-labelling of bacteria

From: David L Haviland (David.L.Haviland@uth.tmc.edu)
Date: Tue Jun 25 2002 - 11:42:54 EST


Mike:

> I have used Molecular Probes Cell Tracker green on viable bacteria for
> cell binding experiments using flow for quant.  This worked very well
> for me.

In your hands, how long did the dye remain detectable?   I'm thinking of a
phagocytosis experiement involving Staph aureus and macrophages, so I need
the bugs to be "detectable" for a long time (>6 hour if not days).   I need
to do some leg work but do you or anyone know if CSFE can be used in
bacteria?

Many thanks
David



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